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These chemicals are produced as we grow generate energy from the food we eat

To detect VDR, we utilized a specific primary antibody (Abcam, ab109234, 1:50 dilution), followed by incubation with an Alexa Fluor-conjugated secondary goat anti-rabbit antibody (Invitrogen, A32731, 1:200 dilution)

After 4 weeks of treatment, the rats from group A-D were euthanized using ketamine

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These results indicate that RSMC is required for Sororincohesin association during S phase

Arrows added in purple to show the direction of gene changes for key enzymes involved in this pathway following melatonin treatment